Difference between revisions of "Part:BBa K619891:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The prfA-UTR parent contruct BBa-K619889 has a RBS which has been optimized for E. coli. | + | The prfA-UTR parent contruct BBa-K619889 has a RBS which has been optimized for E. coli. This version has three mutations, T61A, C67T and A70G. |
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===Source=== | ===Source=== | ||
Latest revision as of 04:14, 29 September 2011
Mutated prfA thermosensor from listeria
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prfA-UTR parent contruct BBa-K619889 has a RBS which has been optimized for E. coli. This version has three mutations, T61A, C67T and A70G.
Source
mutated prfA-UTR