Difference between revisions of "Part:BBa K619890:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | see BBa_K619889, the Shine-Delagarno was mutated from GGGGGA to GGAGGA in order to optimize expression in E. coli, otherwise the thermosensor is the original sequence of prfA-UTR from Listeria monocytogenes. | + | see BBa_K619889, the Shine-Delagarno was mutated from GGGGGA to GGAGGA in order to optimize expression in E. coli, otherwise the thermosensor is the original sequence of prfA-UTR from Listeria monocytogenes. This construct has three mutations, A46G, T56C and T72A |
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===Source=== | ===Source=== | ||
Latest revision as of 04:00, 29 September 2011
mutated prfA thermosensor from listeria
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
see BBa_K619889, the Shine-Delagarno was mutated from GGGGGA to GGAGGA in order to optimize expression in E. coli, otherwise the thermosensor is the original sequence of prfA-UTR from Listeria monocytogenes. This construct has three mutations, A46G, T56C and T72A
Source
This is the prfA-UTR from Listeria monocytogenes.