Difference between revisions of "Part:BBa K511003"

Revision as of 03:54, 29 September 2011

UAS-Gal4 Promoter MammoBlock

This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise.

As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 126
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 126
Illegal NheI site found at 154
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 126
Illegal XhoI site found at 83
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 126
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 126
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K511003 short</partinfo>
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 As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.
+https://static.igem.org/mediawiki/2011/thumb/6/66/Hef1a-GV16%2C_UAS-Reporter_Data.jpg/800px-Hef1a-GV16%2C_UAS-Reporter_Data.jpg
 <!-- Add more about the biology of this part here