Difference between revisions of "Part:BBa K640002:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, rolling circle replication of one of the plasmid strands, and mediated transfer to the receptor organism.
+
oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, host cell polymerases facilitate rolling circle replication of one of the plasmid strands in the host, and <i>rep</i> genes mediate transfer to the receptor organism. This oriT part is an alternate form of BBa_J01003, and allows DNA transfer between <i>E. coli</i> and <i>Pseudomonas</i>.
  
 
===Source===
 
===Source===
  
 
===References===
 
===References===

Revision as of 03:40, 29 September 2011

oriT - Interspecies origin of transfer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, host cell polymerases facilitate rolling circle replication of one of the plasmid strands in the host, and rep genes mediate transfer to the receptor organism. This oriT part is an alternate form of BBa_J01003, and allows DNA transfer between E. coli and Pseudomonas.

Source

References