Difference between revisions of "Part:BBa K620000"

Revision as of 03:29, 29 September 2011

DDT Dehydrochlorinase

Also known as Glutathione S-transferase 1-1, this protein is supposed to degrade DDT, an endocrine disruptor and persistent organic pollutant. The protein was identified from the Anopheles dirus mosquito by [http://www.sciencedirect.com/science/article/pii/0965174895000909 Prapanthadara et al] and the gene was assembled from the protein's amino acid sequence, taken from [http://www.ncbi.nlm.nih.gov/protein/Q93113.1 NCBI's protein database], via PIPE cloning.

Usage and Biology

Plasmid map of K620000 in pSB1C3 (submission plasmid)

Plasmid map of K620000 in pET11-a to amplify and purify the protein.

DDT in reaction buffer, original peak at 235g/mol

DDT reaction with DDT Dehydrochlorinase analyzed with GCMS. There is a peak at 191g/mol and a peak at 206 g/mol

DDT Molecular Structure

DDT structure after GCMS with a molecular weight of 235 g/mole

DDT structure after reaction with DDT Dehydrochlorinase and GCMS with a molecular weight of 206g/mole

Sequence and Features