Difference between revisions of "Part:BBa K648123:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Mutation of active RecA at two different amino acids to attempt to prevent RecA from repairing damaged DNA but not other functions | + | Mutation of active RecA at two different amino acids (Arg 243 and Lys 286) to attempt to prevent RecA from repairing damaged DNA but not other functions |
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===Source=== | ===Source=== | ||
Latest revision as of 02:58, 29 September 2011
RecA with mutation of Lys 286 and Arg 243
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 127
Illegal AgeI site found at 631 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation of active RecA at two different amino acids (Arg 243 and Lys 286) to attempt to prevent RecA from repairing damaged DNA but not other functions
Source
RecA 1 DH10B BBa_648100