Difference between revisions of "Part:BBa K566030"

 
 
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<partinfo>BBa_K566030 short</partinfo>
 
<partinfo>BBa_K566030 short</partinfo>
  
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CcaS gene (constitutive expression)
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CcaS gene with constitutive expression.
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Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:28, 29 September 2011

CcaS gene (constitutive expression)

CcaS gene with constitutive expression.

Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1299
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 670
    Illegal SpeI site found at 1394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1299
    Illegal XhoI site found at 2316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
    Illegal AgeI site found at 1334
  • 1000
    COMPATIBLE WITH RFC[1000]