Difference between revisions of "Part:BBa K542004"

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Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br>
 
Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br>
The microcompartment portion to the "Co-localization Construct".  
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The microcompartment portion to the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
 
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.  
 
This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.  

Latest revision as of 01:59, 29 September 2011

pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD

Made by assembling BBa_K542001 with BBa_K542003.

Note that the pLacI promoter (BBa_R0010) is regulating the Lumazine Synthase gene, not the pBAD promoter (BBa_I13453) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
The microcompartment portion to the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]