Difference between revisions of "Part:BBa K542002"

Latest revision as of 01:58, 29 September 2011

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).

Intermediate part to assemble the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 735
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 675
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <br><br>
 Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br><br>
-Intermediate part to assemble the "Co-localization Construct".
+Intermediate part to assemble the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
 <br><br>
 This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.