Difference between revisions of "Part:BBa K566023"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | It is the best characterized member of the ferredoxin-dependent bilin reductase family. Unlike other ferredoxin-dependent bilin reductases that catalyze a two-electron reduction, PcyA sequentially reduces D-ring (exo) and A-ring (endo) vinyl groups of biliverdin IXalpha (BV) to yield phycocyanobilin, a key pigment precursor of the light-harvesting antennae complexes of red algae, cyanobacteria, and cryptophytes. | |
[[Image:Captura_de_pantalla_2011-09-28_a_las_19.11.40.png|thumb|center|500px|''' | [[Image:Captura_de_pantalla_2011-09-28_a_las_19.11.40.png|thumb|center|500px|''' |
Latest revision as of 01:32, 29 September 2011
pcyA gene (constitutive expression)
pcyA (Phycocyanobilin:ferredoxin oxidoreductase) transcribed from constitutive promoter.
Gene in charge of the cromophore synthesis along with ho1 in Synechocystis which converts heme into PCB via a two-step oxidation/reduction process.
Usage and Biology
It is the best characterized member of the ferredoxin-dependent bilin reductase family. Unlike other ferredoxin-dependent bilin reductases that catalyze a two-electron reduction, PcyA sequentially reduces D-ring (exo) and A-ring (endo) vinyl groups of biliverdin IXalpha (BV) to yield phycocyanobilin, a key pigment precursor of the light-harvesting antennae complexes of red algae, cyanobacteria, and cryptophytes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
- 1000COMPATIBLE WITH RFC[1000]