Difference between revisions of "Part:BBa K566023:Design"
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<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
 
<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
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<b>2.</b> Fukuyama K. <i>et al</i> (2010) Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA). <i>J Biol Chem.</i> <b>285</b>(2): 1000–1007.
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Revision as of 01:19, 29 September 2011

pcyA gene (constitutive expression)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the project that this part was made, it was needed to use other Assambly Standard.


Source

CDS was extracted by PCR, promoter and terminator were Classic BioBricks.

References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324. 2. Fukuyama K. et al (2010) Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA). J Biol Chem. 285(2): 1000–1007.