Difference between revisions of "Part:BBa K569010:Design"

(→‎Source)
(→‎Design Notes)
 
Line 14: Line 14:
 
             foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
 
             foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
 
             reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 
             reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
−
   CheZ  
+
   CheZ*(mutant)
 
           5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3'  
 
           5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3'  
 
           5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'
 
           5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'

Latest revision as of 01:08, 29 September 2011

ctx+CheZ mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 818


Design Notes

We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) 
from an old backbone to add the BioBrick restriction enzymes sites.



 CTX
           foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
           reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 CheZ*(mutant) 
          5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 
          5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'

Source

CheZ*(mutant) --Kindly given by Dr. Ruth Silversmith from the University of North Carolina School of Medicine.

J07011(MIT)

References