Difference between revisions of "Part:BBa K569010:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
  We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) from an old backbone
+
  We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant)  
 +
from an old backbone to add the BioBrick restriction enzymes sites.
 +
 
  
  
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   CheZ  
 
   CheZ  
 
           5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3'  
 
           5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3'  
−
           5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'  
+
           5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'
−
          to add the BioBrick restriction enzymes sites.
+
  
 
===Source===
 
===Source===

Revision as of 01:05, 29 September 2011

ctx+CheZ mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 818


Design Notes

We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) 
from an old backbone to add the BioBrick restriction enzymes sites.



 CTX
           foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
           reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 CheZ 
          5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 
          5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'

Source

Na

References