Difference between revisions of "Part:BBa K569006:Design"

 
(→‎Design Notes)
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__NOTOC__
 
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<partinfo>BBa_K569006 short</partinfo>
 
<partinfo>BBa_K569006 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
NA
+
We designed and used primers to PCR out both the CTX from CTX-GFP and Chey from an old backbone
−
 
+
  
 +
        CTX
 +
            foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
 +
            reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'       
 +
        CheY
 +
            foward-5'-ccatctagacacctgcaaaaggagatgg-3'
 +
            revers-5'-ccaactagtcacctgcaaaaagggacat-3'
  
 
===Source===
 
===Source===

Revision as of 00:52, 29 September 2011

ctx+CheY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 428
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 428
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 428
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed and used primers to PCR out both the CTX from CTX-GFP and Chey from an old backbone 

       CTX
            foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
            reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'        
       CheY 
            foward-5'-ccatctagacacctgcaaaaggagatgg-3'
            revers-5'-ccaactagtcacctgcaaaaagggacat-3'

Source

From iGEM parts

References