Difference between revisions of "Part:BBa K542002"

Revision as of 00:43, 29 September 2011

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).

Intermediate part to assemble the "Co-localization Construct".

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 735
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 675
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 Intermediate part to assemble the "Co-localization Construct".
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-This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct. The data sheet for BBa_K542008 is shown below.
+This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
 <br><br>
 [[image:uoflECdatasheet.png|150px]]