Difference between revisions of "Part:BBa K654059"
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<partinfo>BBa_K654059 short</partinfo> | <partinfo>BBa_K654059 short</partinfo> | ||
| − | Fatty Acyl-CoA Synthetase (Ligase) | + | FadD Fatty Acyl-CoA Synthetase (Ligase) from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The FadD Fatty Acyl-CoA Synthetase (Ligase) converts fatty acids into fatty acyl-CoA using ATP. |
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| + | [[Image:2011_Utah_State_FattyAcylCoASynthetase.png | border | center | 300px]]<br> | ||
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| + | Fatty Acyl-CoA Synthetase is used in conjunction with Thioesterase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.<br><br> | ||
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| + | [[Image:2011_Utah_State_Pathway_2.png | border | center | 700px]]<br> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 00:29, 29 September 2011
Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag
FadD Fatty Acyl-CoA Synthetase (Ligase) from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The FadD Fatty Acyl-CoA Synthetase (Ligase) converts fatty acids into fatty acyl-CoA using ATP.
Fatty Acyl-CoA Synthetase is used in conjunction with Thioesterase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]