Difference between revisions of "Part:BBa K654058"

Revision as of 00:26, 29 September 2011

Thioesterase (TesA from E. coli) with 8-His Tag

TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The TesA thioesterase converts fatty acyl-ACP into fatty acids with a hydration reaction.

Thioesterase is used in conjunction with Fatty Acyl-CoA Synthetase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K654058 short</partinfo>
-TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction.
+TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The TesA thioesterase converts fatty acyl-ACP into fatty acids with a hydration reaction.
-Reaction Catalyzed:
-[[Image:2011_Utah_State_Thioesterase.png]]
-Part of the Following Pathways:
+[[Image:2011_Utah_State_Thioesterase.png | border | center | 300px]]<br>
-[[Image:2011_Utah_State_Pathway_1.png] Fatty Alcohol Production Pathway]
-[[Image:2011_Utah_State_Pathway_2.png] Wax Ester Production Pathway]
+Thioesterase is used in conjunction with Fatty Acyl-CoA Synthetase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.<br><br>
+<br>
+[[Image:2011_Utah_State_Pathway_2.png | border  | center | 700px]]<br>
 <!-- Add more about the biology of this part here