Difference between revisions of "Part:BBa K569012:Design"
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===Source=== | ===Source=== | ||
| − | ToxR-- | + | ToxR-- J07009 (MIT) |
FGFR-- Received from Openbiosystems (Receptor 1: isoform 15) | FGFR-- Received from Openbiosystems (Receptor 1: isoform 15) | ||
===References=== | ===References=== | ||
Latest revision as of 00:16, 29 September 2011
ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1225
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 803
Illegal NgoMIV site found at 1394 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
1. Added restriction sites XbaI and SpeI via PCR mechanism
2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
ToxR-- J07009 (MIT)
FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)