Difference between revisions of "Part:BBa K569013:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K569013 short</partinfo>
 
<partinfo>BBa_K569013 short</partinfo>
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===Design Notes===
 
===Design Notes===
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'''FGFR'''
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      1. Added restriction sites XbaI and SpeI via PCR mechanism
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      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
  
 
===Source===
 
===Source===

Revision as of 00:08, 29 September 2011

PyeaR+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1500
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR 

      1. Added restriction sites XbaI and SpeI via PCR mechanism
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

?

References