Difference between revisions of "Part:BBa K512000"
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<partinfo>BBa_K512000 short</partinfo> | <partinfo>BBa_K512000 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | One of the cas genes responsible for CRISPR interference to work. cas3 has been described as a protein essential to the functionality of the Cascade (CRISPR-associated complex for antiviral defence) complex. | ||
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+ | Brouns, S.J.J., et. al. (2008) Small CRISPR RNAs guide antiviral defense in prokaryotes. ''Science'' '''321''': 960-964. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K512000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K512000 SequenceAndFeatures</partinfo> | ||
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− | References: | + | ===References=== |
+ | #Brouns, S.J.J., et. al. (2008) Small CRISPR RNAs guide antiviral defense in prokaryotes. ''Science'' '''321''': 960-964. |
Revision as of 00:06, 29 September 2011
cas3
Usage and Biology
One of the cas genes responsible for CRISPR interference to work. cas3 has been described as a protein essential to the functionality of the Cascade (CRISPR-associated complex for antiviral defence) complex.
Brouns, S.J.J., et. al. (2008) Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321: 960-964.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1964
Illegal PstI site found at 2302 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1581
Illegal PstI site found at 1964
Illegal PstI site found at 2302 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1964
Illegal PstI site found at 2302 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1964
Illegal PstI site found at 2302 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 214
Illegal SapI site found at 904
References
- Brouns, S.J.J., et. al. (2008) Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321: 960-964.