Difference between revisions of "Part:BBa K569012:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
?
+
ToxR-- Received from iGEM, part J07009
 +
 
 +
FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)
  
 
===References===
 
===References===

Revision as of 00:05, 29 September 2011

ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1225
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 803
    Illegal NgoMIV site found at 1394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR 

      1. Added restriction sites XbaI and SpeI via PCR mechanism
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

ToxR-- Received from iGEM, part J07009

FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)

References