Difference between revisions of "Part:BBa K569012:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | '''FGFR''' | ||
| + | -1. Added restriction sites XbaI and SpeI via PCR mechanism | ||
| + | -2. Did a point mutation experiment because there was an EcoRI restriction site within the gene | ||
===Source=== | ===Source=== | ||
Revision as of 00:04, 29 September 2011
ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1225
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 803
Illegal NgoMIV site found at 1394 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
-1. Added restriction sites XbaI and SpeI via PCR mechanism
-2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
?