Difference between revisions of "Part:BBa K569014:Design"

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ToxR-- Received from iGEM, part J07009
 
ToxR-- Received from iGEM, part J07009
  
−
FGFR-- Received from Openbiosystems
+
FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)
 
    
 
    
 
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119
 
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119
  
 
===References===
 
===References===

Revision as of 00:02, 29 September 2011

SCP+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR 

    - 1. Added restriction sites XbaI and SpeI via PCR mechanism
    - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

ToxR-- Received from iGEM, part J07009

FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)

Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119

References