Difference between revisions of "Part:BBa K569014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | FGFR | + | '''FGFR''' |
- 1. Added restriction sites XbaI and SpeI via PCR mechanism | - 1. Added restriction sites XbaI and SpeI via PCR mechanism | ||
- 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene | - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene | ||
Revision as of 00:00, 29 September 2011
SCP+ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 844
Illegal NgoMIV site found at 1435 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
- 1. Added restriction sites XbaI and SpeI via PCR mechanism
- 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
?