Difference between revisions of "Part:BBa K569014:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
FGFR-- 1. Added restriction sites XbaI and SpeI via PCR mechanism
+
FGFR - 1. Added restriction sites XbaI and SpeI via PCR mechanism
−
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
+
    - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
  
 
===Source===
 
===Source===

Revision as of 23:59, 28 September 2011

SCP+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR - 1. Added restriction sites XbaI and SpeI via PCR mechanism 

    - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

?

References