Difference between revisions of "Part:BBa K569014:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 10: Line 10:
  
 
FGFR-- Received from Openbiosystems
 
FGFR-- Received from Openbiosystems
−
       Added restriction sites XbaI and SpeI via PCR mechanism
+
       1. Added restriction sites XbaI and SpeI via PCR mechanism
−
       Did a point mutation experiment because there was an EcoRI restriction site within the gene
+
       2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
  
 
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119
 
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119

Revision as of 23:58, 28 September 2011

SCP+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ToxR-- Received from iGEM, part J07009

FGFR-- Received from Openbiosystems 

      1. Added restriction sites XbaI and SpeI via PCR mechanism
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119

Source

?

References