Difference between revisions of "Part:BBa K566012:Design"
 
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<b>2.</b> Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. <i>J Bacteriol</i> 189:298–304.
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<b>2.</b> Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. <i>J Bacteriol</i> <b>189</b>:298–304.
 
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Latest revision as of 22:52, 28 September 2011

cI repressor from Lambda phage optimized for E. coli cI (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites for classic BioBricks was performed, they were detected and eliminated.


Source

Sequence was obtained form BioBrick BBa_C0052, it was optimized changing for preferential codons as possible and then synthesized.

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.

2. Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. J Bacteriol 189:298–304.