Difference between revisions of "Part:BBa K653001:Design"
Ernestopro (Talk | contribs) (→Source) |
Ernestopro (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K653001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K653001 SequenceAndFeatures</partinfo> | ||
| − | |||
===Design Notes=== | ===Design Notes=== | ||
| Line 12: | Line 11: | ||
The assembling of this two parts was performed using the 3A Assembly Method. | The assembling of this two parts was performed using the 3A Assembly Method. | ||
| − | |||
| − | |||
| − | |||
Revision as of 22:48, 28 September 2011
GFP reporter plus Double Terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect.
The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable.
The assembling of this two parts was performed using the 3A Assembly Method.
Source
This BBa_E0040part was derived from the reporter device used by Elowitz's repressilator [1]. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers [2] to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia [3]. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.
The BBa_B0015 Double Terminator part is the most commonly used terminator. It seems to be reliable. Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination. The part was designed by Reshma Shetty