Difference between revisions of "Part:BBa K566011"
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Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in ''E. coli''.
 
Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in ''E. coli''.
  
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===Usage and Biology===
 
===Usage and Biology===
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Repressor protein cI from bacteriophage 434. cI434 has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM434 promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM434.
  
 
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Revision as of 22:25, 28 September 2011

cI434 repressor from phage 434 optimized for E. coli cI (+LVA)

Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in E. coli.

Usage and Biology

Repressor protein cI from bacteriophage 434. cI434 has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM434 promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM434.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 436
    Illegal AgeI site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]