Difference between revisions of "Part:BBa K656010:Design"
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''Anabaena'' separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars. | ''Anabaena'' separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars. | ||
| − | The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis. | + | The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis. A similar approach has been shown to successfully limit heterologous expression to vegetative cells in another species of cyanobacteria, Nostoc punctiforme. [1] |
===Source=== | ===Source=== | ||
Revision as of 22:04, 28 September 2011
Anabaena pSac Promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Anabaena separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars.
The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis. A similar approach has been shown to successfully limit heterologous expression to vegetative cells in another species of cyanobacteria, Nostoc punctiforme. [1]
Source
Anaebaena PCC 7120
References
"Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products" http://aem.asm.org/cgi/content/abstract/76/11/3462
Archer, CT et al. The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli. BMC Genomics 12:9 (2011)
Lee, SY and Ho Nam Chang. High cell density cultivation of Escherichia coli W using sucrose as a carbon source. Biotechnology Letters 15:9 (1993) 971--974.
Lee JS, Lee SY and Sunwon Park. Fed-batch culture of Escherichia coli W by exponential feeding of sucrose as a carbon source. Biotechnology Techniques 11:1 (1997) 59-62.
Shukla, VB et al. Production of D(-)-lactate from sucrose and molasses. Biotechnology Letters 26 (2004) 689-693.
Zhang, X et al. Production of L-alanine by metabolically engineered Escherichia coli. Appl Microbiol Biotechnol 77 (2007) 355-366.