Difference between revisions of "Part:BBa K566008:Design"
 
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===References===
 
===References===
  
−
Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro
 
 
<b>1.</b> Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. <i>J. Mol. Riol</i>. (1987) 195, 311-322
 
<b>1.</b> Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. <i>J. Mol. Riol</i>. (1987) 195, 311-322

Latest revision as of 19:55, 28 September 2011

Mnt repressor optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and removed.


Source

Original sequence was taken from BioBrick BBa_C0072, this was optimized with preferential codons for better expression into E. coli.

References

1. Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. J. Mol. Riol. (1987) 195, 311-322