Difference between revisions of "Part:BBa K590014"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 7: | Line 7: | ||
This is a medium copy plasmid backbone that confers kanamycin resistance. It was deposited in the registry with an insert coding for [https://parts.igem.org/wiki/index.php?title=Part:BBa_I7107 LacI-repressible GFP]. | This is a medium copy plasmid backbone that confers kanamycin resistance. It was deposited in the registry with an insert coding for [https://parts.igem.org/wiki/index.php?title=Part:BBa_I7107 LacI-repressible GFP]. | ||
| − | + | <center>https://static.igem.org/mediawiki/2011/d/d1/Washington_pGAefficiency_summary.jpg</center> | |
Below is the gel image of the plasmid amplified with universal pGA backbone primers [https://parts.igem.org/Part:BBa_K590086 pGAsuffix_fwd] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590084 pGAprefix_rev]. The band is near the expected length of 2750 bp. | Below is the gel image of the plasmid amplified with universal pGA backbone primers [https://parts.igem.org/Part:BBa_K590086 pGAsuffix_fwd] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590084 pGAprefix_rev]. The band is near the expected length of 2750 bp. | ||
Revision as of 19:53, 28 September 2011
pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
This is a Gibson Cloning friendly 3K3 plasmid backbone that was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] as part of the [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly toolkit].
Usage and Biology
This is a medium copy plasmid backbone that confers kanamycin resistance. It was deposited in the registry with an insert coding for LacI-repressible GFP.
Below is the gel image of the plasmid amplified with universal pGA backbone primers pGAsuffix_fwd and pGAprefix_rev. The band is near the expected length of 2750 bp.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal NheI site found at 1384 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal BglII site found at 2744
Illegal BamHI site found at 1
Illegal XhoI site found at 16
Illegal XhoI site found at 177
Illegal XhoI site found at 1020 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal AgeI site found at 1470
Illegal AgeI site found at 1793 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2498