Difference between revisions of "Part:BBa K590014"

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===Usage and Biology===
 
===Usage and Biology===
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This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP.
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This is a medium copy plasmid backbone which has kanamycin resistance, with a LacI-repressible promoter that drives GFP expression.
  
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Below is the gel image of the plasmid backbone with 3555bp in length:
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Below is the gel image of the plasmid amplified with universal pGA backbone primers [https://parts.igem.org/Part:BBa_K590086 pGAsuffix_fwd] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590084 pGAprefix_rev]. The band is near the expected length of 2750 bp.
 
[[Image:Igem2011 3k3 placGFP gel.png|thumb|center|1kb Ladder (left), pGA3k3 (right)]]
 
[[Image:Igem2011 3k3 placGFP gel.png|thumb|center|1kb Ladder (left), pGA3k3 (right)]]
  

Revision as of 19:13, 28 September 2011

pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

This is a Gibson Cloning friendly 3K3 plasmid backbone that was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] as part of the [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly toolkit].

Usage and Biology

This is a medium copy plasmid backbone which has kanamycin resistance, with a LacI-repressible promoter that drives GFP expression.

Below is the gel image of the plasmid amplified with universal pGA backbone primers pGAsuffix_fwd and pGAprefix_rev. The band is near the expected length of 2750 bp.

1kb Ladder (left), pGA3k3 (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2729
    Illegal NheI site found at 1384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2729
    Illegal BglII site found at 2744
    Illegal BamHI site found at 1
    Illegal XhoI site found at 16
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2729
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2498