Difference between revisions of "Part:BBa K566004"
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Protein generator of cyan fluorescent protein without transcription terminator. It may be negatively regulated by Mnt repressor ([https://parts.igem.org/Part:BBa_K566002 BBa_C0072]). | Protein generator of cyan fluorescent protein without transcription terminator. It may be negatively regulated by Mnt repressor ([https://parts.igem.org/Part:BBa_K566002 BBa_C0072]). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i>Aequorea victoria</i> . It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1. | + | Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea victoria </i>. It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Modified with an SsrA tail for rapid degradation of the protein and faster fall time for the emission. |
Revision as of 19:13, 28 September 2011
CFP protein generator without transcription terminator
Protein generator of cyan fluorescent protein without transcription terminator. It may be negatively regulated by Mnt repressor (BBa_C0072).
Usage and Biology
Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Modified with an SsrA tail for rapid degradation of the protein and faster fall time for the emission.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 39
Illegal XhoI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 107
- 1000COMPATIBLE WITH RFC[1000]