Difference between revisions of "Part:BBa K542009"
 
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Made by assembling [https://parts.igem.org/Part:BBa_J04500 BBa_J04500] with [https://parts.igem.org/Part:BBa_K346007 BBa_K346007].
 
Made by assembling [https://parts.igem.org/Part:BBa_J04500 BBa_J04500] with [https://parts.igem.org/Part:BBa_K346007 BBa_K346007].
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Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS ([https://parts.igem.org/Part:BBa_K346007 BBa_K346007]). The pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) and RBS ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]) was added.
 
Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS ([https://parts.igem.org/Part:BBa_K346007 BBa_K346007]). The pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) and RBS ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]) was added.
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The Peking 2010 iGEM team characterized Antigen 43 (AG43) using a T7 promoter expression system in <i>E. coli</i> BL21(DE3) strain and submitted the coding sequence with no promoter or ribosome binding site (RBS) to the parts registry (BBa_K364007). In order for AG43 to be used, BBa_K542009 was assembled to include the required regulatory elements. The pLacI promoter and RBS were added to the coding sequence for AG43.
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Datasheet for BBa_K542009 in <i>E. coli</i> strain BL21 (DE3). You may also wish to refer to the "Experience" page.
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[[image:uoflag43datasheet.png|150px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:08, 28 September 2011

pLacI Regulated AG43

Made by assembling BBa_J04500 with BBa_K346007.

Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS (BBa_K346007). The pLacI promoter (BBa_R0010) and RBS (BBa_B0034) was added.

The Peking 2010 iGEM team characterized Antigen 43 (AG43) using a T7 promoter expression system in E. coli BL21(DE3) strain and submitted the coding sequence with no promoter or ribosome binding site (RBS) to the parts registry (BBa_K364007). In order for AG43 to be used, BBa_K542009 was assembled to include the required regulatory elements. The pLacI promoter and RBS were added to the coding sequence for AG43.

Datasheet for BBa_K542009 in E. coli strain BL21 (DE3). You may also wish to refer to the "Experience" page.

Uoflag43datasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 2159
    Illegal NgoMIV site found at 2669
    Illegal NgoMIV site found at 2690
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2429
    Illegal AgeI site found at 2843
    Illegal AgeI site found at 3085
  • 1000
    COMPATIBLE WITH RFC[1000]