Difference between revisions of "Part:BBa K542003"

Revision as of 18:47, 28 September 2011

pBAD Regulated TetR

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")

Intermediate part to assemble the "Co-localization Construct".

Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440].
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 Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")
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