Difference between revisions of "Part:BBa K542003"
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Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440]. | Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440]. | ||
| − | Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")<br> | + | Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off") |
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Intermediate part to assemble the "Co-localization Construct". | Intermediate part to assemble the "Co-localization Construct". | ||
| + | <br><br> | ||
| + | Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct. | ||
| + | <br><br> | ||
| + | [[image:uoflECdatasheet.png|150px]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:47, 28 September 2011
pBAD Regulated TetR
Made by assembling BBa_I13453 with BBa_P0440.
Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")
Intermediate part to assemble the "Co-localization Construct".
Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]