Difference between revisions of "Part:BBa K566006"
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===Usage and Biology===
 
===Usage and Biology===
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This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0073 BBa_R0073]); so that only when green light is ON and any other Mnt source is OFF, expression from pMnt is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.  
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This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0073 BBa_R0073]); so that only when green light is ON and any other Mnt source is OFF, expression from the Mnt repressible promoter is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.  
  
 
  [[Image:AND.png|thumb|center|700px|'''Figure 1. Repression based AND-gate.'''  Mnt repressor, which acts on the gene of interest, is expressed from two repressible promoters: the PenI repressible promoter and a different promoter. PenI repressor is placed under pCpcG2 control, so that it is stimulated by green light. A second input must control the expression of a second repressor, which represses the second Mnt source. Only when both green light and the second input are ON, the two Mnt sources are OFF and the gene of interest can be expressed.]]
 
  [[Image:AND.png|thumb|center|700px|'''Figure 1. Repression based AND-gate.'''  Mnt repressor, which acts on the gene of interest, is expressed from two repressible promoters: the PenI repressible promoter and a different promoter. PenI repressor is placed under pCpcG2 control, so that it is stimulated by green light. A second input must control the expression of a second repressor, which represses the second Mnt source. Only when both green light and the second input are ON, the two Mnt sources are OFF and the gene of interest can be expressed.]]

Revision as of 16:45, 28 September 2011

penI-pCpcG2-pPenI-mnt

Repression-based potential AND gate. Mnt Repressor is under the control of the PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light(1).

Usage and Biology

This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter (BBa_R0073); so that only when green light is ON and any other Mnt source is OFF, expression from the Mnt repressible promoter is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.

Figure 1. Repression based AND-gate. Mnt repressor, which acts on the gene of interest, is expressed from two repressible promoters: the PenI repressible promoter and a different promoter. PenI repressor is placed under pCpcG2 control, so that it is stimulated by green light. A second input must control the expression of a second repressor, which represses the second Mnt source. Only when both green light and the second input are ON, the two Mnt sources are OFF and the gene of interest can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]