Difference between revisions of "Part:BBa K569017:Design"
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Latest revision as of 16:28, 28 September 2011
CheY
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 275
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 275
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 275
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 275
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone.
Source
K422007 ETHZ_Basel 2010