Difference between revisions of "Part:BBa K569017:Design"

 
(Design Notes)
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<partinfo>BBa_K569017 short</partinfo>
 
<partinfo>BBa_K569017 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone.
 
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===Source===
 
===Source===

Revision as of 16:27, 28 September 2011

CheY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 275
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone.

Source

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References