Difference between revisions of "Part:BBa K615004:Design"
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===Design Notes=== | ===Design Notes=== | ||
Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly. | Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly. | ||
| + | |||
| + | Note: two illegal Pst1 sites (bases 2-7 and 812-817) were removed using Quikchange, but those sites have not yet been sequence verified. | ||
Latest revision as of 16:01, 28 September 2011
Zif268 binding site-His3-URA3 construct for one-hybrid selection
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 15
Illegal BglII site found at 528
Illegal BglII site found at 588
Illegal BamHI site found at 818 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1508
Illegal SapI.rc site found at 1355
Design Notes
Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly.
Note: two illegal Pst1 sites (bases 2-7 and 812-817) were removed using Quikchange, but those sites have not yet been sequence verified.
Source
The sequence was synthesized.