Difference between revisions of "Part:BBa K542011:Design"

 
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The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]).
 
The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]).
  
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*David Lain Houston, PhD Student
 
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Institute of Cellular, Molecular, and Systems Biology
 
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Susan Rosser Group
 
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Arnott Lab
 
  
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David Lain Houston*, PhD Student<br>
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Institute of Cellular, Molecular, and Systems Biology<br>
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Susan Rosser Group<br>
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Arnott Lab<br>
 
University of Glasgow
 
University of Glasgow
  
 
===References===
 
===References===

Latest revision as of 12:35, 28 September 2011

Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 483
    Illegal AgeI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal.


Source

The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added (BBa_K249005).


David Lain Houston*, PhD Student
Institute of Cellular, Molecular, and Systems Biology
Susan Rosser Group
Arnott Lab
University of Glasgow

References