Difference between revisions of "Part:BBa K566013:Design"
 
Line 16: Line 16:
 
==References==
 
==References==
  
−
<b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell science at a glance</i> <b>5</b>:837-838
+
<b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell Science at a Glance</i> <b>5</b>:837-838

Latest revision as of 08:10, 28 September 2011

RFP optimized for E. coli (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was design considering use of preferential codons, as well, a complete search looking for illegal sites for classic BioBricks was performed, they were found and deleted.


Source

Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell Science at a Glance 5:837-838