Difference between revisions of "Part:BBa K566013:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K566013 short</partinfo>
 
<partinfo>BBa_K566013 short</partinfo>
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Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.  
 
Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.  
  
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===References===
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==References==
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<b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell science at a glance</i> <b>5</b>:837-838

Revision as of 08:09, 28 September 2011

RFP optimized for E. coli (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was design considering use of preferential codons, as well, a complete search looking for illegal sites for classic BioBricks was performed, they were found and deleted.


Source

Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell science at a glance 5:837-838