Difference between revisions of "Part:BBa K566010:Design"
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| + | <b>1.</b> Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of <i>Bacillus licheniformis</i>. <i>J Bacteriol</i> <b>175</b>:7383-7390 | ||
Latest revision as of 07:14, 28 September 2011
PenI repressor optimized for E. coli (inverted sequence)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, they were found and eliminated.
Source
Original sequence was taken from Registry (BBa_C0074), this was optimized with preferential codons for better expression in E. coli.
References
1. Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of Bacillus licheniformis. J Bacteriol 175:7383-7390