Difference between revisions of "Part:BBa K566006:Design"

 
 
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===References===
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==References==
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<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324.
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Latest revision as of 05:40, 28 September 2011

penI-pCpcG2-pPenI-mnt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and removed.


Source

Sequence of pCpcG2 promoter was obtained from pJT122 plasmid of Dr. Jeff Tabor. Rest of the sequences were obtained from the Registry.

Complete construction was synthesized.


References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.