Difference between revisions of "Part:BBa K566004:Design"
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==References==
 
==References==
  
−
<p> 1. Patterson G., Rich N. Day and David P.(2001) Fluorescent protein spectra. Cell science at a glance 5:837-838</p>
+
<p> 1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell science at a glance 5:837-838</p>

Revision as of 05:33, 28 September 2011

CFP protein generator without transcription terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for better expression in E. coli. Illegal sites were removed.


Source

Synthesized DNA.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell science at a glance 5:837-838