Difference between revisions of "Part:BBa K566003:Design"
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==References==
 
==References==
  
−
<p> 1. Patterson G., Rich N. Day and David P.(2001 Fluorescent protein spectra. Cell science at a glance 5:837-838</p>
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<p> 1. Patterson G., Rich N. Day and David P.(2001) Fluorescent protein spectra. Cell science at a glance 5:837-838</p>

Revision as of 05:28, 28 September 2011

CFP optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preferential codons were implemented for improving expression in E. coli.

Analysis of illegal sites was made, they were found and removed.


Source

Synthesized DNA.


References

1. Patterson G., Rich N. Day and David P.(2001) Fluorescent protein spectra. Cell science at a glance 5:837-838