Difference between revisions of "Part:BBa K548000"

Revision as of 02:18, 28 September 2011

Humanized Aequorin

This BioBrick is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity and oxidizes its substrate, coelenterazine to emit blue light (λmax=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed. The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 62
Illegal NgoMIV site found at 226
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K548000 short</partinfo>
-This is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity. And oxidizes its substrate, Coelenterazine to emit blue light (&#955;max=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed.  The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.
+This BioBrick is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity and oxidizes its substrate, coelenterazine to emit blue light (&#955;max=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed.  The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.
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