Difference between revisions of "Part:BBa K566000"
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===Usage and Biology===
 
===Usage and Biology===
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OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda ([https://parts.igem.org/Part:BBa_K566000 1]). Its binding sites maybe used to design of new regulatory networks or to mimic Lambda's natural behavior.  
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OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda ([https://parts.igem.org/Part:BBa_K566000 1]). Its binding sites maybe used to design new regulatory networks or to mimic Lambda's natural behavior.  
  
 
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Revision as of 01:33, 28 September 2011

OL region from Lambda

OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).

Usage and Biology

OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda (1). Its binding sites maybe used to design new regulatory networks or to mimic Lambda's natural behavior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]